Hsp90 Antibody : FITC (OASE00053-FITC)

Catalog No: OASE00053-FITC

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Bovine, Chicken, Pig, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: D7A
• Isotype: IgG
• Host: Mouse
• Conjugation: FITC: Fluorescein Isothiocyanate
• Application: IP, WB, ELISA, IHC
• Additional Information: Background Info: Recognizes 90kDa proteins corresponding to the molecular mass of Hsp90. Hsp90alpha specific for human samples. Can isolate complexes of Hsp90, Src kinase and cec37.
  Scientific Background: Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (4-7). Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90- regulated proteins that have been discovered to date are involved in cell signaling (8-9). The number of proteins now known to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase(6). When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immune-adsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function (10).
  Certificate of Analysis: 2ug/ml was sufficient for detection of Hsp90alpha in 20ug of heat shocked HeLa cell lysate as well as in 100ng oh human Hsp90alpha protein by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Full length protein Hsp90 purified from chicken brain
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1:500 (WB), 5ug with 20uL Protein A beads (IP )
• Storage Buffer: PBS, 50% glycerol
• Reference: 1. Schuh S. et al. (1985) J Biol Chem. 260 (26): 14292-14296.
  2. Lipsich L.A., Cutt J.R. and Brugge J.S. (1982) Mol. Cell Biol. 2(7): 875-880.
  3. Brugge J.S., Yonemoto W., and Darrow D. (1983) Mol. Cell. Biol. 3(1): 9-19.
  4. Arlander SJH, et al. (2003) J Biol Chem 278: 52572-52577.
  5. Pearl H, et al. (2001) Adv Protein Chem 59: 157-186.
  6. Neckers L, et al. (2002) Trends Mol Med 8:S55-S61.
  7. Pratt W, Toft D. (2003) Exp Biol Med 228:111-133.
  8. Pratt W, Toft D. (1997) Endocr Rev 18: 306–360.
  9. Pratt WB. (1998) Proc Soc Exptl Biol Med 217: 420–434.
  10. Whitesell L, et al. (1994) Proc Natl Acad Sci USA 91: 8324– 8328.

Target Info

• Gene Symbol: BAZ1B
• Gene Full Name: bromodomain adjacent to zinc finger domain, 1B
• Alias Symbols: WSTF, WBSCR9, WBSCR10
• NCBI Gene Id: 9031
• Uniprot ID: P11501
• Protein Accession #: NP_001103255.1