Hsp90 Antibody : Biotin (OASE00020-Biotin)

Catalog No: OASE00020-Biotin

Size:200ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Rabbit, Chicken, Achyla, Wheat Germ, Sf9 cell line
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: AC-16
• Isotype: IgG2b
• Host: Mouse
• Conjugation: Biotin
• Application: WB, IHC
• Additional Information: Background Info: This antibody is reactive with both the constitutive and the inducible form of Hsp90. It does not bind to the native form and does not recognize Hsp90 from E.coli or yeast.
  Scientific Background: Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (1-4). Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90-regulated proteins that have been discovered to
  date are involved in cell signaling (5-6). The number of proteins now know to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5 When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation.
  In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immuno-adsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function (7).
  Certificate of Analysis: 1 ug/mL was sufficient for detection of Hsp90 in 20ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Heat shock protein 900 from the water mold Achyla ambisexualis
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1ug/ml was sufficient for detection of hsp90 by Western Blot in 20ug of HeLa lysate.
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Arlander SJH, et al. (2003) J Biol Chem 278: 52572-52577. 2. Pearl H, et al. (2001) Adv Protein Chem 59:157-186.
  3. Neckers L, et al. (2002) Trends Mol Med 8:S55-S61.
  4. Pratt W, Toft D. (2003) Exp Biol Med 228:111-133.
  5. Pratt W, Toft D. (1997) Endocr Rev 18: 306–360.
  6. Pratt WB. (1998) Proc Soc Exptl Biol Med 217: 420–434.
  7. Whitesell L, et al. (1994) Proc Natl Acad Sci USA 91: 8324–
  8328.

Target Info

• Gene Symbol: HSP90AB1
• Gene Full Name: heat shock protein 90kDa alpha (cytosolic), class B member 1
• Alias Symbols: HSP84, HSPC2, HSPCB, D6S182, HSP90B
• NCBI Gene Id: 3326
• Uniprot ID: Q8LLI5
• Protein Accession #: NP_031381.2