Size:100ug
$370.00
Discontinued
SKU
OASE00049
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OASE00049-HRP Conjugated

OASE00049-Biotin Conjugated

Hsp90 alpha/beta Antibody (OASE00049)

Catalog#: OASE00049
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This product is Genway GWB-E26A2E
More Information
Predicted Species Reactivity Human, Rat, Liver, S. cerevisiae, S. pombe
Clonality Monoclonal
Clone Hyb-K41220A
Isotype IgG2a
Host Mouse
Application WB, IHC
Additional Information Background Info: Detects 90kD proteins corresponding to the molecular mass of hsp90 alpha or beta.
:: Scientific Background: HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms alpha and beta, which share 85% sequence amino acid homology. The two isoforms of Hsp90 are expressed in the cytosolic compartment (1). Despite the similarities, HSP90alpha exists predominantly as a homodimer while HSP90beta exists mainly as a monomer.(2) From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex. (3-6) Furthermore, Hsp90 is highly conserved between species; having 60% and 78% amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively.
Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90-regulated proteins that have been discovered to date are involved in cell signaling (7-8). The number of proteins now know to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5 When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation.
In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function (9).
:: Certificate of Analysis: 1ug/ml was sufficient for detection of Hsp90alphabeta in 20ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Reconstitution and Storage Store at -20C. Shipping in Blue Ice or 4C.
Replacement Item This antibody may replace item sc-1055 from Santa Cruz Biotechnology.
Immunogen Recombinant human Hsp90alpha; Specificity mapped to amino acids 291-304
Purification Protein G Purified
Concentration 1mg/mL
Datasheets/Manuals Printable datasheet for OASE00049
Dilution 1ug/ml was sufficient for detection of hsp90alphabeta by Western Blot in 20ug of HeLa lysate.
Storage Buffer PBS pH7.2, 50% glycerol
Target Reference 1. Nemoto, T. et al. (1997) J.Biol Chem. 272, 26179-26187.
2. Minami, Y, et al. (1991), J.Biol Chem. 266, 10099-10103.
3. Arlander SJH, et al. (2003) J Biol Chem 278, 52572-52577.
4. Pearl H, et al. (2001) Adv Protein Chem 59,157-186.
5. Neckers L, et al. (2002) Trends Mol Med 8:S55-S61.
6. Pratt W, Toft D. (2003) Exp Biol Med 228:111-133.
7. Pratt W, Toft D. (1997) Endocr Rev 18,306–360.
8. Pratt WB. (1998) Proc Soc Exptl Biol Med 217, 420–434.
9. Whitesell L, et al. (1994) Proc Natl Acad Sci USA 91, 8324–8328.
10. Kishimoto J, et al. (2005). Cell Stress and Chaperones. 10 (4), 296-311.
Official Gene Full Name heat shock protein 90kDa alpha (cytosolic), class B member 1; heat shock protein 90kDa alpha (cytosolic), class A member 1
Target HSP90AB1, HSP90AA1
NCBI Gene Id 3326; 3320
Swissprot Id P08238, P07900
Protein Accession # NP_031381.2, NP_001017963.2
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express HSP90AB1. , HSP90AA1.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express HSP90AB1. , HSP90AA1.
  1. What is the species homology for "Hsp90 alpha/beta Antibody (OASE00049)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Rat, Liver, S. cerevisiae, S. pombe".

  2. How long will it take to receive "Hsp90 alpha/beta Antibody (OASE00049)"?

    This item is available "Domestic: within 3-5 days delivery | International: 3-5 days".

  3. What buffer format is "Hsp90 alpha/beta Antibody (OASE00049)" provided in?

    This item is provided in "".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Hsp90 alpha/beta Antibody (OASE00049)"?

    This target may also be called "" in publications.

  5. What is the shipping cost for "Hsp90 alpha/beta Antibody (OASE00049)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Hsp90 alpha/beta Antibody (OASE00049)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Hsp90 alpha/beta Antibody (OASE00049)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Hsp90 alpha/beta Antibody (OASE00049)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for "HSP90AB1, HSP90AA1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "HSP90AB1, HSP90AA1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "HSP90AB1, HSP90AA1"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "HSP90AB1, HSP90AA1"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "HSP90AB1, HSP90AA1"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "HSP90AB1, HSP90AA1"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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