Hsp90 alpha/beta Antibody (OASE00048)
This product is Genway GWB-FC7950
|Predicted Species Reactivity||Human, Rat, Liver, S. cerevisiae, S. pombe|
|Additional Information||Background Info: Detects 90kD proteins corresponding to the molecular mass of hsp90 alpha or beta.|
|::||Scientific Background: HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms alpha and beta, which share 85% sequence amino acid homology. The two isoforms of Hsp90 are expressed in the cytosolic compartment (1). Despite the similarities, HSP90alpha exists predominantly as a homodimer while HSP90beta exists mainly as a monomer.(2) From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex. (3-6) Furthermore, Hsp90 is highly conserved between species; having 60% and 78% amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively.
Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90-regulated proteins that have been discovered to date are involved in cell signaling (7-8). The number of proteins now know to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5 When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation.
In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function (9).
|::||Certificate of Analysis: 1ug/ml was sufficient for detection of Hsp90alphabeta in 20ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.|
|Reconstitution and Storage||Store at -20C. Shipping in Blue Ice or 4C.|
|Replacement Item||This antibody may replace item sc-1055 from Santa Cruz Biotechnology.|
|Immunogen||Recombinant human Hsp90alpha; Specificity mapped to amino acids 291-304|
|Purification||Protein G Purified|
|Datasheets/Manuals||Printable datasheet for OASE00048|
|Dilution||1ug/ml was sufficient for detection of hsp90alphabeta by Western Blot in 20ug of HeLa lysate.|
|Storage Buffer||PBS pH7.2, 50% glycerol|
|Target Reference||1. Nemoto, T. et al. (1997) J.Biol Chem. 272, 26179-26187.
2. Minami, Y, et al. (1991), J.Biol Chem. 266, 10099-10103.
3. Arlander SJH, et al. (2003) J Biol Chem 278, 52572-52577.
4. Pearl H, et al. (2001) Adv Protein Chem 59,157-186.
5. Neckers L, et al. (2002) Trends Mol Med 8:S55-S61.
6. Pratt W, Toft D. (2003) Exp Biol Med 228:111-133.
7. Pratt W, Toft D. (1997) Endocr Rev 18,306–360.
8. Pratt WB. (1998) Proc Soc Exptl Biol Med 217, 420–434.
9. Whitesell L, et al. (1994) Proc Natl Acad Sci USA 91, 8324–8328.
10. Kishimoto J, et al. (2005). Cell Stress and Chaperones. 10 (4), 296-311.
|Official Gene Full Name||heat shock protein 90kDa alpha (cytosolic), class B member 1; heat shock protein 90kDa alpha (cytosolic), class A member 1|
|NCBI Gene Id||3326; 3320|
|Swissprot Id||P08238, P07900|
|Protein Accession #||NP_031381.2, NP_001017963.2|
|Tissue Tool||Find tissues and cell lines supported by DNA array analysis to express HSP90AB1. , HSP90AA1.|
|RNA Seq||Find tissues and cell lines supported by RNA-seq analysis to express HSP90AB1. , HSP90AA1.|
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
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