HSP73 Recombinant Protein (Bovine) (OPED00064)

Catalog No: OPED00064

Size:100 ug

SDS-PAGE analysis: Lane 1: MW Marker, Lane 2: 1ug, Lane 3: 2ug, Lane 4: 5ug HSC70/HSP73 (bovine), (recombinant) (biotin conjugate).

Product Info

• Predicted Species Reactivity: Bovine
• Product Format: Liquid. In 10mM sodium phosphate, pH 7.2.
• Application: WB
• Additional Information: The Hsp70 family of heat shock proteins contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
• Reconstitution and Storage: Store at -80C
• Purification: Purified by multi-step chromatography.
• Purity: >90% (SDS-PAGE; Western blot)
• Application Info: Western blot control.
• Reference: Heat shock protein 70 interacts with aquaporin-2 and regulates its trafficking: D. Brown, et al. ; J. Biol. Chem. 282, 28721 (2007), Application(s): GST Pulldown , Abstract;
  Metabolically biotinylated adenovirus for cell targeting, ligand screening, and vector purification: M.A. Barry, et al. ; Mol. Ther. 8, 688 (2003), Application(s): In Vitro Assay , Abstract;

Target Info

• Gene Symbol: HSPA8
• Gene Full Name: heat shock protein 8
• Alias Symbols: Hsc70, HSPA10
• NCBI Gene Id: 281831
• Protein Name: Heat shock cognate protein
• Description of Target: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex (By similarity). Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase CHIP (By similarity).
• Uniprot ID: P19120
• Protein Accession #: NP_006258.3
• Nucleotide Accession #: NM_006267.4
• Protein Size (# AA): 650
• Molecular Weight: 73 kDa