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HSP70 ELISA Kit (Gallus) (OKCD04259)

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Predicted Species Reactivity:
Gallus
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
HSP70
Alias Symbols:
Heat shock 70 kDa protein, HSP70
Protein Name:
Heat shock protein 70
Swissprot Id:
Q7SX63
Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
0.55 ng/mL
Kit Range:
1.56-100 ng/mL
Kit Reproducibility:
Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level HSP70. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level HSP70 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology HSP70 ELISA Kit (Gallus) (OKCD04259) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSP70 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSP70 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSP70 captured in the well.
Kit Component:
ComponentAmount
Hsp70 Microplate96 Wells (12 x 8 Well strips)
Hsp70 Lyophilized Standard2
100X Hsp70 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Linearity:
Sample1:21:41:81:16
Serum (n=5)90-101%79-93%92-101%89-97%
EDTA Plasma (n=5)94-102%95-103%80-92%86-103%
Heparin Plasma (n=5)96-105%79-105%92-101%88-99%
Kit Recovery:
MatrixRecovery range (%)Average (%)
Serum (n=5)98-105102
EDTA Plasma (n=5)96-103101
Heparin Plasma (n=5)79-10581
Kit Detection Method:
Colorimetric, OD 450 nm
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA

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