Hsp60 Antibody : HRP (OASE00018-HRP)

Catalog No: OASE00018-HRP

Size:200ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Bovine, Canine, Chicken, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Spinach, Ecoli, H pylori, S typhimurium, T spiralis, yeast, white fly
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Monoclonal
• Clone: LK2
• Isotype: IgG1
• Host: Mouse
• Conjugation: HRP: Horseradish Peroxidase
• Application: WB, IHC, FC
• Additional Information: Background Info: Detects an ~60kDa protein corresponding to the molecular mass of Hsp60 on SDS PAGE immunoblots.
  Scientific Background: In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. Hsp60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian Hsp60 (1-3). Whereas mammalian Hsp60 is localized within the mitochondria, plant Hsp60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts.
  
  It has been indicated that these proteins carry out a very important biological function due to the fact that Hsp60 is present in so many different species. The common characteristics of the Hsp60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, Hsp60 with its co-chaperonin, Hsp10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of Hsp60-Hsp10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of Hsp60 and Hsp10 is their protective functions against infection and cellular stress. Hsp60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).
  
  Certificate of Analysis: 0.25ug/mL was sufficient for detection of Hsp60 in10ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Recombinant human Hsp60
• Purification: Protein G Purified
• Concentration: 0.6-0.7 mg/ml
• Dilution: 0.25ug/mL (WB)
• Storage Buffer: PBS, 50% glycerol
• Reference: 1. Hartl, F.U. (1996) Nature 381: 571-579.
  2. Bukau, B. and Horwich, A.L. (1998) Cell 92: 351-366.
  3. Hartl, F.U. and Hayer-Hartl, M. (2002) Science 295: 1852- 1858.
  4. Jindal, S., et al. (1989) Molecular and Cellular Biology 9: 2279-2283.
  5. La Verda, D., et al (1999) Infect Dis. Obstet. Gynecol. 7: 64-71.
  6. Itoh, H. et al. (2002) Eur. J. Biochem. 269: 5931-5938.
  7. Gupta, S. and Knowlton, A.A. J. Cell Mol Med. 9: 51-58.
  8. Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11: 116-128.
  9. Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292: E292-E297.
  10. Gao, Y.L., et al (1995) J. of Immunology 154: 3548-3556.
  11. Neuer, A., et al (1997) European Society for Human Reproduction and Embryology 12(5):925-929.
  12. Bason, C., et al (2003) Lancet 362(9400): 1971-1977.
  

Target Info

• Gene Symbol: HSPD1
• Gene Full Name: heat shock 60kDa protein 1 (chaperonin)
• Alias Symbols: HLD4, CPN60, GROEL, HSP60, HSP65, SPG13, HSP-60, HuCHA60
• NCBI Gene Id: 3329
• Uniprot ID: P10809
• Protein Accession #: NP_002147.2