Hsp40, YDJ1 Antibody : HRP (OASE00071-HRP)

Catalog No: OASE00071-HRP

Size:100ug

Product Info

• Predicted Species Reactivity: Yeast. Does not cross react with Human, Mouse or Rat.
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Monoclonal
• Clone: 1G10.H8
• Isotype: IgG1 Kappa
• Host: Mouse
• Conjugation: HRP: Horseradish Peroxidase
• Application: WB, IP, ELISA
• Additional Information: Background Info: Detects ~40kDa.
  Scientific Background: Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). There are two main types of Hsp40; type 1 DNAJ proteins including HDJ2 and yeast YdjI; type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. (3, 4), type II does not.
  Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. Hsp40 stimulates the ATPase activity of Hsp70 which in turn causes conformational changes of the unfolded proteins (5, 6). The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and
  HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (7).
  Certificate of Analysis: 0.5 ug/mL was sufficient for detection of 50ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Full length protein yeast Hsp40 (YDJ1)
• Purification: Protein G Purified
• Concentration: 0.6-0.7 mg/ml
• Dilution: 1:2000 (WB)
• Storage Buffer: 50% glycerol
• Reference: 1. Cheetham M.E. and Caplan A.J. (1998) Cell Stress Chaperones 3: 28–36.
  2. Fan C.Y., et al. (2003) Cell Stress Chaperones 8: 309–316.
  3. Terda K., et al. (1997) J Cell Biol. 139: 1089-1095.
  4. Lu Z. and Cyr D.M. (1998) J Biol Chem. 273: 27824-27830.
  5. Liberek K. et al. (1991) Proc. Natl. Acad. Sci. USA 88: 2874–2878.
  6. Cyr D.M., et al. (1992) J Biol Chem. 267: 20927–20931.
  7. Höhfeld J., et al. (2001) EMBO Rep. 2: 885–890.

Target Info

• Gene Symbol: YDJ1
• Alias Symbols: HSP40, MAB3, MAS5
• NCBI Gene Id: 855661
• Uniprot ID: P25491
• Protein Accession #: NP_014335.1