Hsp40, Hdj1 Antibody (OASE00065)

Catalog No: OASE00065 (Formerly GWB-709CD1)

Size:100ug

Western Blot analysis of Human Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 3B9.E6. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 3B9.E6. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone: 3B9.E6
• Isotype: IgG1
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, IHC, ICC, IF, IP, ELISA
• Additional Information: Background Info: Detects ~40kDa. Does not cross-react with HDJ2 or YDJ1.
  Scientific Background: Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of Hsp70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).
  Certificate of Analysis: 0.5 ug/mL was sufficient for detection of Hsp40 (HDJ1) in 15ug of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Recombinant Protein HSP40 (Hdj1)
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~40kDa. Does not cross-react with HDJ2 or YDJ1.
• Dilution: WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS pH7.2, 50% glycerol, 0.09% sodium azide
• Reference: 1. Cheetham M.E. and Caplan A.J. (1998) Cell Stress Chaperones 3: 28–36.
  2. Fan C.Y., et al. (2003) Cell Stress Chaperones 8: 309–316.
  3. Sohn S.Y., Kim S.B., Kim J., and Ahn B.Y. (2006) J Gen Virol. 87(7): 1883-91.
  4. Liberek K. et al. (1991) Proc. Natl. Acad. Sci. USA 88: 2874–2878.
  5. Cyr D.M., et al. (1992) J Biol Chem. 267: 20927–20931.
  6. Höhfeld J., et al. (2001) EMBO Rep. 2: 885–890.
  7. Terda K., et al. (1997) J Cell Biol. 139: 1089-1095.
  8. Lu Z. and Cyr D.M. (1998) J Biol Chem. 273: 27824-27830.
• Description: Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human HSP40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of HSP70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).

Target Info

• Gene Symbol: HSP40
• Gene Full Name: DnaJ (Hsp40) homolog, subfamily B, member 1
• Alias Symbols: DNAJ1, NDAJB1, HDJ1, HSP40, HSPF1, DnaJ homolog subfamily B member 1, Dna J protein homolog 1, Heat shock 40 kDa protein 1, HSP40, heat shock protein 40, Human DnaJ protein 1, hDj-1
• NCBI Gene Id: 3337
• Uniprot ID: P25685
• Protein Accession #: NP_006136.1