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Hsp40 Antibody (OASE00326)

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Gene Symbol:
DNAJB1
Official Gene Full Name:
DnaJ (Hsp40) homolog, subfamily B, member 1
NCBI Gene Id:
3337
Swissprot Id:
P25685
Protein Accession #:
NP_006136.1
Replacement Item:
This antibody may replace item sc-114001 from Santa Cruz Biotechnology.
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Rabbit antiserum
Application:
WB, IP
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express DNAJB1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express DNAJB1.
Immunogen:
Recombinant purified human hsp40
Predicted Species Reactivity:
Human
Reconstitution and Storage:
Store at -20C. Shipping in Blue Ice or 4C.
Datasheets/Manuals:
Printable datasheet for OASE00326
Dilution:
1: 1500-2,000 (WB)
Additional Information:
Background Info: Detects an ~40kDa protein corresponding to the molecular mass of Hsp40 on SDS PAGE immunoblots. Also detects recombinant Hsp40.
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Scientific Background: DnaJ/Hsp40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, Hsp70s. Because the ATP hydrolysis is essential for the activity of Hsp70s, DnaJ/Hsp40 proteins actually determine the activity of Hsp70s by stabilizing their interaction with substrate proteins. DnaJ/Hsp40 proteins all contain the J domain through which they bind to Hsp70s.
Hsp40, also known as HDJ1 (6), is a basic mammalian 40kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (2-5). Hsp 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli; an intracellular pattern similar to Hsc70/Hsp70, the mammalian homologues of the bacterial heat shock protein, DnaK (2).
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Certificate of Analysis: 0.5 ug/mL was sufficient for detection of Hsp40 in 20ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Target Reference:
1. Melville, M. W. et al. (1997) PNAS USA, 94: 97-102. 2. Hattori, H., Liu, Y-C., Tohnai, I., Ueda, M., Kaneda, T., Kobayashi, T., Tanabe, K., and Ohtsuka, K. (1992) Cell Structure and Function 17: 77-86.
3. Ohtsuka, K. Masuda, A., Nakai, A., and Nagata, K. (1990) Biochem. Biophys. Res. Commun. 166: 642-647.
4. Bardwell, J.C.A., Tilly, K., Craig, E., King, J., Zylicz, M. and Georgopoulos, C. (1986) J. Biol. Chem. 261: 1782-1785.
5. Ohku, M., Tamura, F., Nishimura, S., and Uchida, H. (1986) J. Biol. Chem. 261: 1778-1781.
6. Ohtsuka, K. (1993) Biochem. Biophys. Res. Commun. 197: 235-240.
Storage Buffer:
Rabbit antiserum

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