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- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for OASE00091-FITC |
|---|
| Predicted Species Reactivity | Human. Very limited cross-reactivity to other species. |
|---|---|
| Product Format | Liquid. Purified antibody supplied in 1x PBS buffer. |
| Clonality | Monoclonal |
| Clone | 5D12-A12 |
| Isotype | IgG2b Kappa |
| Host | Mouse |
| Conjugation | FITC: Fluorescein Isothiocyanate |
| Application | WB, IP, ELISA, IHC |
| Additional Information | Background Info: Detects ~27kDa proteins corresponding to Hsp27 on SDS Page Immunoblots. Has no cross-reactivity to alphaB crystallin. |
| :: | Scientific Background: Hsp27s belong to an abundant and ubiquitous family of Hsp27s belong to an abundant and ubiquitous family of |
| :: | Certificate of Analysis: 0.5 ug/mL was sufficient for detection of Hsp27 in 10ug of HeLa lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
| Immunogen | Human Hsp27 |
| Purification | Protein G Purified |
| Concentration | 1 mg/ml |
| Dilution | 0.25-0.5 ug/mL (WB) |
| Storage Buffer | PBS pH7.4, 50% glycerol |
| Reference | 1. Kim K.K., Kim R., and Kim, S. (1998) Nature 394(6693): 595-599. 2. Van Montfort R., Slingsby C., and Vierling E. (2001) Addv Protein Chem. 59: 105-56. 3. Ehrnsperger M., Graber S., Gaestel M. and Buchner J. (1997) EMBO J. 16: 221-229. 4. Ciocca D.R., Oesterreich S., Chamness G.C., McGuire W.L., and Fugua S.A. (1993) J Natl Cancer Inst. 85 (19): 1558-70. 5. Sarto C., Binnz P.A., and Mocarelli P. (2000) Electrophoresis. 21(6): 1218-26. 6. Arrigo A.P. (2005) J Cell Biochem. 94(2): 241-6. |
| Gene Symbol | HSPB1 |
|---|---|
| Gene Full Name | heat shock 27kDa protein 1 |
| Alias Symbols | CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102 |
| NCBI Gene Id | 3315 |
| Uniprot ID | P04792 |
| Protein Accession # | NP_001531.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Hsp27 Antibody : FITC (OASE00091-FITC)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human. Very limited cross-reactivity to other species.".
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How long will it take to receive "Hsp27 Antibody : FITC (OASE00091-FITC)"?
This item is available "Domestic: within 3-5 days delivery | International: 3-5 days".
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What buffer format is "Hsp27 Antibody : FITC (OASE00091-FITC)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Hsp27 Antibody : FITC (OASE00091-FITC)"?
This target may also be called "CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102" in publications.
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What is the shipping cost for "Hsp27 Antibody : FITC (OASE00091-FITC)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Hsp27 Antibody : FITC (OASE00091-FITC)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Hsp27 Antibody : FITC (OASE00091-FITC)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Hsp27 Antibody : FITC (OASE00091-FITC)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "HSPB1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "HSPB1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "HSPB1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "HSPB1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "HSPB1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "HSPB1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
