Hsp22 Antibody : HRP (OASE00134-HRP)

Catalog No: OASE00134-HRP

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Monoclonal
• Clone: 3C12-H11
• Isotype: IgG1 Kappa
• Host: Mouse
• Conjugation: HRP: Horseradish Peroxidase
• Application: WB, IHC, ELISA, IF
• Additional Information: Background Info: Detects endogenous and exogenous hsp22 in monomeric, dimeric and tetrameric forms in WB. Does not cross react with alpha crystallin.
  Scientific Background: Hsp22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and
  the human protein is most closely related to Hsp27.
  Similar to most other sHSPs, Hsp22 is predominately
  transcribed in skeletal muscle and heart, as well as the
  placenta (1). Hsp22 is a monomeric protein which
  interacts with HSPB1. It displays temperature-dependent
  chaperone activity.
  In a two hybrid screen, HspB8 interacted preferentially
  with a triple aspartate form of Hsp27 which mimics
  Hsp27 phosphorylated at Ser15, Ser78, and Ser82, as
  compared to wild-type Hsp27 (2). HSPB8 has two binding
  domains (N and C Terminal) that are specific for
  different binding partners, and has the ability to bind
  itself and other sHSPs (3). The chaperone-like activity is
  of great importance to the function of Hsp22 in various
  processes including proliferation, apoptosis and
  macro autophagy (4).
  Mutations in the HSPB8 gene are associated with the
  inherited peripheral neuropathies, autosomal dominant distal
  hereditary motor neuropathy type IIA (dSMA) and axonal
  Charcot-Marie-Tooth disease type 2L (CMT2L) (5).
  Certificate of Analysis: 1 ug/mL was sufficient for detection of Hsp22 in 20 ug of whole rat tissue extract by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: His-tagged human recombinant hsp22
• Purification: Protein G Purified
• Concentration: 0.6-0.7 mg/ml
• Dilution: 1:2000 (WB)
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1.Kappe G., et al. (2001) Biochem Biophys Acta 1520: 1-6.
  2.Benndorf R., et al. (2001) J Biol Chem 276: 26753-
  26761.
  3.Sun X., et al. (2004) J Biol Chem 279: 2394-2402.
  4.Kim M.V., et al. (2004) Biochem Biophys Res Commun
  325: 649-652.
  5. Wilhelmus M.M., et al. (2006)Acta Neuropathol (Berl)
  111: 139-149.

Target Info

• Gene Symbol: GNL3
• Gene Full Name: guanine nucleotide binding protein-like 3 (nucleolar)
• Alias Symbols: NS, E2IG3, NNP47, C77032
• NCBI Gene Id: 26354
• Uniprot ID: Q9UJY2
• Protein Accession #: NP_055180.2