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Catalog No: OKEH01893
Size:96T
Price: $625.00
SKU
OKEH01893
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology HSF4 ELISA Kit (Human) (OKEH01893) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSF4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSF4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSF4 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.1% (n = 20)
Mean Inter-assay CV%: < 9.8% (n = 20)
ELISA Kit Component
ComponentAmount
HSF4 Microplate96 Wells (12 x 8 Well strips)
HSF4 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated HSF4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.076 ng/mL
SpecificityNatural and recombinant Human Heat shock factor protein 4
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHSF4
Gene Full Nameheat shock transcription factor 4
Alias SymbolsCTM;CTRCT5;heat shock factor protein 4;Heat shock transcription factor 4;hHSF4;HSF 4;HSTF 4.
NCBI Gene Id3299
Protein NameQ9ULV5
Description of TargetHeat shock factor protein 4
Uniprot IDhttps://www.uniprot.org/uniprot/Q9ULV5
Protein Accession #NP_001035757.1
Nucleotide Accession #NM_001040667.2
Protein Size (# AA)492
Molecular Weight53 kDa
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