HSF2 Antibody (OASE00029)

Catalog No: OASE00029 (Formerly GWB-FA5258)

Size:25ug

Western Blot analysis of Human K562 cell lysates showing detection of HSF2 protein using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2. Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody at 1:1000. Cells transiently transfected with control, HSF1 or HSF2 shRNA constructs. Courtesy of: Lea Sistonen, Abo Akademi University, Finland.

Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2. Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF2 Antibody. (C) Composite. Heat Shocked at 42C for 1h.

Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2. Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF2 Antibody. (C) Composite. Heat Shocked at 42C for 1h.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Guinea Pig, Hamster, Monkey, Rabbit, Dog, Bovine, Sheep, Pig
• Clonality: Monoclonal
• Clone: 3E2
• Isotype: IgG1
• Host: Rat
• Conjugation: Unconjugated
• Application: WB, ICC, IF, AM
• Additional Information: Background Info: Detects an ~69kDa protein in unstressed cells corresponding to the molecular mass HSF2 on SDS PAGE immunoblots.
  Scientific Background: HSF2, or heat shock factor 2, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6).
  There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).
  
  Certificate of Analysis: 4 ug/mL was sufficient for detection of HSF2 in 20ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Purified recombinant mouse HSF2 protein
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~69kDa.
• Dilution: WB (1:250), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Reference: 1. Cotto J.J., Fox S.G. and Morimoto R.I. (1997) J. Cell Science 110: 2925-2934.
  2. Morano K.A. and Thiele D.J. (1999). Gene Expression 7 (6): 271-82.
  3.Tanaka KI et al. (2007). JBC Papers Online Manuscript M704081200.
  4. Morimoto R. I. (1998) Genes Dev 12: 3788-3796.
  5. McMillan D. R., Xiao X., Shao L., Graves K., and Benjamin I. J. (1998) J Bio Chem 273: 7523-7528.
  6. Jolly C., Usson Y. and Morimoto R.I. (1999) Proc. Natl. Acad. Sci. USA 96 (12): 6769- 6774.
  7. Fiorenza M.T., Farkas T., Dissing M., Kolding D. and Zimarino V. (1995) Nucleic Acids Res. 23 (3):467-474.
  8. Goodson M.L., Park-Sarge O.K. and Sarge K.D. (1995) Mol. Cell. Biol. 15(10): 5288-5293.
  9. Rallu M., et al. (1997) Proc. Natl. Acad. Sci. USA 94(6): 2392-2397.
  10. Sarge K.D., et al. (1994) Biol. Reprod. 50(6): 1334- 1343.
  11. Murphy S.P., Gorzowski J.J., Sarge K.D. and Phillips B. (1994) Mol. Cell. Biol. 14(8):5309-5317.
• Description: HSF2, or heat shock factor 2, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).

Target Info

• Gene Symbol: HSF2
• Gene Full Name: heat shock factor 2
• Alias Symbols: HSTF2, Heat shock factor protein 2, Heat shock transcription factor 2, HSF 2
• NCBI Gene Id: 15500
• Uniprot ID: P38533 
• Protein Accession #: NP_001129036.1