HSF1 Antibody : FITC (OASE00322-FITC)

Catalog No: OASE00322-FITC

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Bovine, Guinea Pig, Hamster, Monkey, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: 10H4
• Isotype: IgG1
• Host: Rat
• Conjugation: FITC: Fluorescein Isothiocyanate
• Application: WB, IP, ICC, ELISA, GS
• Additional Information: Background Info: Detects an ~85kDa protein in unstressed cell lysates, and an ~95kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms of HSF1 on SDS-PAGE immunoblots.
  Scientific Background: HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6).
  There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).
  
  Certificate of Analysis: 1 ug/mL was sufficient for detection of HSF1 in 20ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Purified recombinant mouse HSF1 protein, epitope mapping to amino acids 378-395
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1:1000 WB
• Storage Buffer: PBS pH 7.4, 50% glycerol, 0.1% sodium azide
• Reference: 1. Cotto J.J., Fox S.G. and Morimoto R.I. (1997) J. Cell Science 110: 2925-2934.
  2. Morano K.A. and Thiele D.J. (1999). Gene Expression 7 (6): 271-82.
  3. Tanaka K.I., et al. (2007). JBC Papers Online Manuscript M704081200.
  4. Morimoto R. I. (1998) Genes Dev 12: 3788-3796.
  5. McMillan D. R., et al. (1998) J Bio Chem 273: 7523-7528.
  6. Jolly C., Usson Y. and Morimoto R.I. (1999) Proc. Natl. Acad. Sci. USA 96 (12): 6769- 6774.
  7. Fiorenza M.T., et al. (1995) Nucleic Acids Res. 23 (3):467-474.
  8. Goodson M.L., Park-Sarge O.K. and Sarge K.D. (1995) Mol. Cell. Biol. 15(10): 5288-5293.
  9. Rallu M., et al. (1997) Proc. Natl. Acad. Sci. USA 94(6): 2392-2397.
  10. Sarge K.D., et al. (1994) Biol. Reprod. 50(6): 1334-1343.
  11. Murphy S.P., Gorzowski J.J., Sarge K.D. and Phillips B. (1994) Mol. Cell. Biol. 14(8):5309-5317.
  

Target Info

• Gene Symbol: HSF1
• Gene Full Name: heat shock transcription factor 1
• Alias Symbols: HSTF1
• NCBI Gene Id: 3297
• Uniprot ID: Q00613
• Protein Accession #: NP_005517.1