HSD17B3 Antibody (OAGA04684)

Catalog No: OAGA04684

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human hepatoma, using HSD17B3 antibody antibody at 1:500 dilution.

HSD17B3 antibody detects HSD17B3 protein at cytoplasm by confocal immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: HSD17B3 protein stained by HSD17B3 antibody diluted at 1:500.
Blue: Hoechst 33342 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid1XPBS, 1% BSA, 20% glycerol (pH 7) with 0.025% Proclin300 as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze/thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human HSD17B3. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1.64 mg/ml
• Application Info: ICC/IF: 1:100~1000
  IHC-P: 1:100~1000
  WB: 1:500~3000

Target Info

• Gene Symbol: HSD17B3
• Gene Full Name: hydroxysteroid 17-beta dehydrogenase 3
• Alias Symbols: EDH17B3, SDR12C2
• NCBI Gene Id: 3293
• Protein Name: testosterone 17-beta-dehydrogenase 3
• Description of Target: This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia.
• Uniprot ID: P37058
• Protein Accession #: NP_000188.1
• Nucleotide Accession #: NM_000197.1
• Molecular Weight: 35 kDa