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Catalog No: OKEH05337 (Formerly GWB-NLN8YR)
Size:96T
Price: $675.00
SKU
OKEH05337
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH05337
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology HSD17B13 ELISA Kit (Human) (OKEH05337) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSD17B13 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSD17B13 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSD17B13 captured in the well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 88%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <8.4%, n = 20
Mean Inter-Assay Precision - <11.1%, n = 20
ELISA Kit Component
ComponentAmount
HSD17B13 Microplate96 Wells (12 x 8 Well strips)
HSD17B13 Lyophilized Standard2
100X Biotinylated HSD17B13 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.156 ng/mL
SpecificityNatural and recombinant Human 17-beta-hydroxysteroid dehydrogenase 13
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHSD17B13
Gene Full Namehydroxysteroid 17-beta dehydrogenase 13
Alias Symbols17-beta hydroxysteroid dehydrogenase;17-beta-HSD 13;17-beta-hydroxysteroid dehydrogenase 13;HMFN0376;NIIL497;SCDR9;SDR16C3;short chain dehydrogenase/reductase family 16C member 3;short-chain dehydrogenase/reductase 9.
NCBI Gene Id345275
Protein NameQ7Z5P4
Description of Target17-beta-hydroxysteroid dehydrogenase 13
Uniprot IDhttps://www.uniprot.org/uniprot/Q7Z5P4
Protein Accession #NP_001129702.1
Nucleotide Accession #NM_001136230.2
Protein Size (# AA)300
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