HSD17B10 ELISA Kit (Human) (OKEH01765)

Catalog No: OKEH01765

Size:96T

Human HSD17B10 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology HSD17B10 ELISA Kit (Human) (OKEH01765) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSD17B10 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSD17B10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSD17B10 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.1% (n = 20)
  Mean Inter-assay CV%: < 8.9% (n = 20)
• ELISA Kit Component:
  • HSD17B10 Microplate: 96 Wells (12 x 8 Well strips)
  • HSD17B10 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated HSD17B10 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.087 ng/mL
• Specificity: Natural and recombinant Human 3-hydroxyacyl-CoA dehydrogenase type-2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HSD17B10
• Gene Full Name: hydroxysteroid 17-beta dehydrogenase 10
• Alias Symbols: 17-beta-hydroxysteroid dehydrogenase 10;17b-HSD10;2-methyl-3-hydroxybutyryl-CoA dehydrogenase;3-hydroxy-2-methylbutyryl-CoA dehydrogenase;3-hydroxyacyl-CoA dehydrogenase type II;3-hydroxyacyl-CoA dehydrogenase type-2;ABAD;AB-binding alcohol dehydrogenase;amyloid-beta peptide binding alcohol dehydrogenase;CAMR;DUPXp11.22;endoplasmic reticulum-associated amyloid beta-peptide-binding protein;ERAB;HADH2;HCD2;HSD10MD;MHBD;mitochondrial ribonuclease P protein 2;mitochondrial RNase P subunit 2;MRPP2;MRX17;MRX31;MRXS10;SCHAD;SDR5C1;Short chain dehydrogenase/reductase family 5C member 1;short chain L-3-hydroxyacyl-CoA dehydrogenase type 2;short chain type dehydrogenase/reductase XH98G2;Short-chain type dehydrogenase/reductase XH98G2;Type II HADH.
• NCBI Gene Id: 3028
• Protein Name: Q99714
• Description of Target: 3-hydroxyacyl-CoA dehydrogenase type-2
• Uniprot ID: https://www.uniprot.org/uniprot/Q99714
• Protein Accession #: NP_001032900.1
• Nucleotide Accession #: NM_001037811.2
• Protein Size (# AA): 261
• Molecular Weight: 26 kDa