- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for HSD17B1 Antibody - N-terminal region (OABB00995) |
---|
Tested Species Reactivity | Human, Rat |
---|---|
Predicted Species Reactivity | Hamster|Human|Rat |
Product Format | Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Clonality | Polyclonal |
Clone | Polyclonal |
Isotype | Rabbit IgG |
Host | Rabbit |
Application | Immunocytochemistry|Immunohistochemistry|Western blot |
Additional Information | Notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
:: | Background: Estradiol 17-beta-dehydrogenase 1 is an enzyme that in humans is encoded by the HSD17B1 gene. This gene encodes a member of the 17beta-hydroxysteroid dehydrogenase family of short-chain dehydrogenases/reductases. It has a dual function in estrogen activation and androgen inactivation and plays a major role in establishing the estrogen E2 concentration gradient between serum and peripheral tissues. The encoded protein catalyzes the last step in estrogen activation, using NADPH to convert estrogens E1 and E2 and androgens like 4-androstenedione, to testosterone. It has an N-terminal short-chain dehydrogenase domain with a cofactor binding site, and a narrow, hydrophobic C-terminal domain with a steroid substrate binding site. This gene is expressed primarily in the placenta and ovarian granulosa cells, and to a lesser extent, in the endometrium, adipose tissue, and prostate. Polymorphisms in this gene have been linked to breast and prostate cancer. A pseudogene of this gene has been identified. Alternative splicing results in multiple transcript variants. |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Immunogen | Polypeptide |
Purification | Affinity Purified |
Concentration | 500 ug/ml |
Specificity | No cross reactivity with other proteins. |
Application Info | Western blot: 0.1-0.5 ug/ml: Human, Rat: Mouse Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: Mouse: By Heat Immunocytochemistry : 0.5-1 ug/ml: Human: Mouse, Rat |
Storage Buffer | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Description | Rabbit IgG polyclonal antibody for Estradiol 17-beta-dehydrogenase 1(HSD17B1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat. |
Gene Symbol | HSD17B1 |
---|---|
Gene Full Name | hydroxysteroid 17-beta dehydrogenase 1 |
Alias Symbols | 17-beta-HSD;17-beta-HSD 1;17-beta-hydroxysteroid dehydrogenase type 1;20 alpha-hydroxysteroid dehydrogenase;20-alpha-HSD;E2DH;EDH17B2;EDHB17;estradiol 17-beta-dehydrogenase 1;HSD17;placental 17-beta-hydroxysteroid dehydrogenase;SDR28C1;short chain dehydrogenase/reductase family 28C member 1;short chain dehydrogenase/reductase family 28CE, member 1. |
NCBI Gene Id | 3292 |
Protein Name | Estradiol 17-beta-dehydrogenase 1 |
Description of Target | Favors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH. |
Uniprot ID | P14061 |
Molecular Weight | 34950 MW |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "HSD17B1 Antibody - N-terminal region (OABB00995)"?
The tested species reactivity for this item is "Human, Rat". This antibody is predicted to have homology to "Hamster|Human|Rat".
-
How long will it take to receive "HSD17B1 Antibody - N-terminal region (OABB00995)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
-
What buffer format is "HSD17B1 Antibody - N-terminal region (OABB00995)" provided in?
This item is provided in "Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "HSD17B1 Antibody - N-terminal region (OABB00995)"?
This target may also be called "17-beta-HSD;17-beta-HSD 1;17-beta-hydroxysteroid dehydrogenase type 1;20 alpha-hydroxysteroid dehydrogenase;20-alpha-HSD;E2DH;EDH17B2;EDHB17;estradiol 17-beta-dehydrogenase 1;HSD17;placental 17-beta-hydroxysteroid dehydrogenase;SDR28C1;short chain dehydrogenase/reductase family 28C member 1;short chain dehydrogenase/reductase family 28CE, member 1." in publications.
-
What is the shipping cost for "HSD17B1 Antibody - N-terminal region (OABB00995)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "HSD17B1 Antibody - N-terminal region (OABB00995)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "HSD17B1 Antibody - N-terminal region (OABB00995)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "34950 MW".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "HSD17B1 Antibody - N-terminal region (OABB00995)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "HSD17B1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "HSD17B1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "HSD17B1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "HSD17B1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "HSD17B1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "HSD17B1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.