- Official Gene Full Name:
- heat shock protein 8
- NCBI Gene Id:
- Replacement Item:
- This antibody may replace item sc-137210 from Santa Cruz Biotechnology.
- Rabbit antiserum
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express hspa8.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express hspa8.
- Synthetic peptide conjugated to KLH. The peptide target is specific to the Hsc70 of salmonid fish.
- Predicted Species Reactivity:
- Salmon. There is predicted reactivity to Bovine, Human, Mouse, Rat and Zebrafish.
- Reconstitution and Storage:
- Store at -20C. Shipping in Blue Ice or 4C.
- Printable datasheet for OASE00441
- 1:50,000 for ECL Advance, and 1:5,000 for ECL)
- Additional Information:
- Background Info: The antibody detects Hsc70, but not Hsp70. There is a one amino acid difference in the target between fish and other animals, but the antibody will detect Hsc70 from other animal species.
- Scientific Background: Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (1). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins
and synthetic peptides (2). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (3). The structure of this ATP
binding domain displays multiple features of nucleotide binding proteins (4).
When cells are subjected to metabolic stress (e.g., heat shock) a member of the hsp 70 family, hsp 70 (hsp72), is expressed; hsp 70 is highly related to hsc70 (>90% sequence identity). Constitutively expressed hsc70 rapidly forms a stable complex with the highly inducible hsp70 in cells following heat shock. The interaction of hsc70 with hsp 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on hsc70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (5).
- Target Reference:
- 1. Boorstein W.R., Ziegelhoffer T., and Craig E.A. (1993) J. Mol. Evol. 38(1): 1-17.
2. Rothman J. (1989), Cell 59: 591-601.
3. DeLuca-Flaherty et al. (1990) Cell 62: 875-887.
4. Bork P., Sander C., and Valencia A. (1992) Proc. Nut1
Acad. Sci. USA 89: 7290-7294.
5. Brown C.L. et al. (1996) J. Biol. Chem. 271(2): 833-
- Storage Buffer:
- Lyophilized rabbit antiserum