HS6ST3 ELISA Kit (Human) (OKCD09118)

Catalog No: OKCD09118

Size:96WELLS

HS6ST3 ELISA Kit (Human) (OKCD09118) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparan Sulfate-6-O-Sulfotransferase 3 (HS6ST3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heparan Sulfate-6-O-Sulfotransferase 3 (HS6ST3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparan Sulfate-6-O-Sulfotransferase 3 (HS6ST3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Heparan Sulfate-6-O-Sulfotransferase 3 (HS6ST3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heparan Sulfate-6-O-Sulfotransferase 3 (HS6ST3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heparan Sulfate-6-O-Sulfotransferase 3 (HS6ST3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • HS6ST3 Microplate: 96 Wells (12 x 8 Well strips)
  • HS6ST3 Lyophilized Standard: 2
  • 100X HS6ST3 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.073ng/mL

Target Info

• Gene Symbol: HS6ST3
• Gene Full Name: heparan sulfate 6-O-sulfotransferase 3
• Alias Symbols: HS6ST-3
• NCBI Gene Id: 266722
• Protein Name: Heparan-sulfate 6-O-sulfotransferase 3
• Description of Target: Heparan sulfate (HS) sulfotransferases, such as HS6ST3, modify HS to generate structures required for interactions between HS and a variety of proteins. These interactions are implicated in proliferation and differentiation, adhesion, migration, inflammation, blood coagulation, and other diverse processes.Heparan sulfate (HS) sulfotransferases, such as HS6ST3, modify HS to generate structures required for interactions between HS and a variety of proteins. These interactions are implicated in proliferation and differentiation, adhesion, migration, inflammation, blood coagulation, and other diverse processes (Habuchi et al., 2000.)...
• Uniprot ID: Q8IZP7
• Protein Accession #: NP_703157.2
• Nucleotide Accession #: NM_153456.3