HS6ST1 ELISA Kit (Human) (OKEH07953)

Catalog No: OKEH07953

Size:96T

Human HS6ST1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	95-105% (n = 20)	102-112% (n = 20)	103-116% (n = 20)	98-108% (n = 20)
  EDTA plasma(n=5)	93-103% (n = 20)	90-102% (n = 20)	85-95% (n = 20)	108-117% (n = 20)
  heparin plasma(n=5)	103-113% (n = 20)	87-97% (n = 20)	111-120% (n = 20)	89-99% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology HS6ST1 ELISA Kit (Human) (OKEH07953) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HS6ST1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HS6ST1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HS6ST1 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 117% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.0% (n = 20)
  Mean Inter-assay CV%: <=11.9% (n = 20)
• ELISA Kit Component:
  • HS6ST1 Microplate: 96 Wells (12 x 8 Well strips)
  • HS6ST1 Lyophilized Standard: 2
  • 100X Biotinylated HS6ST1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: 6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate. Critical for normal neuronal development where it may play a role in neuron branching. May also play a role in limb development. May prefer iduronic acid.
  Subcellular Location: Membrane Single-pass type II membrane protein
  Unigene: Hs.512841
  SMR: O60243
  String: 9606.ENSP00000259241
  KEGG: hsa:9394
  MIM: 604846
  Pfam: PF03567
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 20pg/mL
• Specificity: Natural and recombinant Human Heparan-sulfate 6-O-sulfotransferase 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HS6ST1
• Gene Full Name: heparan sulfate 6-O-sulfotransferase 1
• Alias Symbols: heparan-sulfate 6-O-sulfotransferase 1;heparan-sulfate 6-sulfotransferase;HH15;HS6ST;HS6ST-1.
• NCBI Gene Id: 9394
• Protein Name: O60243
• Description of Target: Heparan-sulfate 6-O-sulfotransferase 1
• Uniprot ID: https://www.uniprot.org/uniprot/O60243
• Protein Accession #: NP_004798.3
• Nucleotide Accession #: NM_004807.2