HRP - Horseradish Peroxidase Protein (OPPA00841)

Catalog No: OPPA00841 (Formerly GWB-8221EA)

Size:10MG

Product Info

• Product Format: Lyophilized
  Physical Appearance: Sterile filtered red-brown powder
• Reconstitution and Storage: It is recommended to reconstitute the lyophilized HRP in sterile 18MΩ-cm H2O not less than 100 μg/ml or more than 10 mg/ml solutions.
  Lyophilized HRP although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HRP should be stored at 4°C between 2-7 days and for future use below -18°C.
  Please prevent freeze-thaw cycles.
• Purification: Purified by affinity chromatography, which results in an enzyme of high specific activity and purity.
• Purity: (A403/A275) = RZ: 3.0
• Biological Activity: 276 U/mg (25°C, guaiacol as the hydrogen donor, pH-7 and H₂O₂ as substrates).
• Source: Root extracts of horseradish

Target Info

• Gene Symbol: HRP
• Alias Symbols: Horseradish Peroxidase, HRP, EC 1.11.1.7
• Description of Target: The enzyme horseradish peroxidase, found in horseradish, is used extensively in molecular biologyand in antibody amplification and detection, among other things. For example, "In recent years the technique of marking neurons with the enzyme horseradish peroxidase (HRP) has become a major tool. In its brief history, this method has probably been used by more neurobiologists than have used the Golgi stainsince its discovery in 1870." Horseradish peroxidase is also highly used in techniques such as Western Blot and ELISA.
  HRP is widely used as an enzymatic label in immunoassays. Usually, the enzyme is coupled to antibodies, lectins or haptens. Coupling to antibodies etc. may be performed through the carbohydrate side chains of the HRP.
• Molecular Weight: 44 kDa