HRIHFB2025 Antibody - C-terminal region (OAAB17671)

Catalog No: OAAB17671

Size:400ul

Western blot analysis of HRIHFB2025 Antibody (C-term) (Cat. # OAAB17671) in MDA-MB231 cell line lysates (35ug/lane). HRIHFB2025 (arrow) was detected using the purified Pab.

CBY1 is strongly supported by BioGPS gene expression data to be expressed in MDA-MB231

Formalin-fixed and paraffin-embedded human brain tissue reacted with HRIHFB2025 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

HRIHFB2025 Antibody (C-term) (Cat. # OAAB17671) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC, FC
• Additional Information: Function: Inhibits the Wnt/Wingless pathway by binding to beta- catenin and inhibiting beta-catenin-mediated transcriptional activation through competition with TCF/LEF transcription factors Has also been shown to play a role in regulating the intracellular trafficking of polycystin-2/PKD2 and possibly of other intracellular proteins. Promotes adipocyte and cardiomyocyte differentiation.
  Cellular Location: Nucleus speckle. Golgi apparatus, trans- Golgi network. Note=Nuclear, in a punctate manner. Also found in the trans-Golgi
  Tissue Location: Widely expressed. Expressed at higher levels in heart, skeletal muscle, kidney and placenta. Also found in brain, lung, liver and testis. Significantly down-regulated in thyroid and metastatic uterine tumors
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This HRIHFB2025 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-126 amino acids from the C-terminal region of human HRIHFB2025.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  CBY1 is strongly supported by BioGPS gene expression data to be expressed in MDA-MB231

• Intended Use: HRIHFB2025 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50

Target Info

• Gene Symbol: CBY1
• Alias Symbols: CBY, arb1, PGEA1, C22orf2, Chibby1, PIGEA14, PIGEA-14, HS508I15A
• NCBI Gene Id: 25776
• Protein Name: Protein chibby homolog 1
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q9Y3M2
• Protein Accession #: NP_001002880.2
• Molecular Weight: 14470 Da