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Catalog No: OCOA17224
Size:20UG
Price: $169.00
SKU
OCOA17224
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HRH1 Protein Lysate (OCOA17224)

Datasheets/ManualsPrintable datasheet for HRH1 Protein Lysate (OCOA17224)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionHRH1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolHRH1
Gene Full Namehistamine receptor H1
Alias SymbolsH1R;H1-R;HH1R;hisH1;histamine H1 receptor;histamine receptor, subclass H1.
NCBI Gene Id3269
Protein NameNM_000861, NP_000852, P35367
Description of TargetHistamine H1 receptor, histamine H1 receptor [Homo sapiens], Homo sapiens histamine receptor H1 (HRH1), transcript variant 4, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/P35367
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_000852
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_000861
Molecular Weight55.8 kDa
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