HRH1 Antibody - C-terminal region (OAEB00846)

Catalog No: OAEB00846 (Formerly GWB-D015BB)

Size:100UG

OAEB00846 (4ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: P-ELISA, WB, IHC, IF
• Reconstitution and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Immunogen: HRH1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Peptide Sequence: CNENFKKTFKRILH
• Concentration: 0.5 mg/ml
• Specificity: Variants (NP_000852.1; NP_001091681.1; NP_001091682.1; NP_001091683.1) encode the same protein.
• Application Info: Peptide ELISA: Antibody detection limit dilution 1:32,000.
  Western Blot: Preliminary experiments gave bands at approx 55kDa and 37kDa in Human Duodenum lysate after 0.3 ug/ml antibody staining. Please note that an explanation cannot be currently found in the literature for the 37 kDa band observed given the calculated size of 55.8 kDa according to NP_000852.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional band).
  IHC: In paraffin embedded Human Colon shows membranous staining. Recommended concentration: 4-6 ug/ml. Paraffin embedded Human Small Intestine. Recommended concentration: 5 ug/ml. This product has been successfully used in IHC on Rat
  IF: This product has been successfully used in IF on Rat
  Optimal dilutions should be determined by the end user.
• Reference: This antibody has been successfully used in IHC on Rat:
  Zhuang QX, Li GY, Li B, Zhang CZ, Zhang XY, Xi K, Li HZ, Wang JJ, Zhu JN. Regularizing firing patterns of rat subthalamic neurons ameliorates parkinsonian motor deficits. J Clin Invest. 2018 Sep 18. PMID: 30226827
  This antibody has been successfully used in IF on Rat:
  Chen ZP, Zhang XY, Peng SY, Yang ZQ, Wang YB, Zhang YX, Chen X, Wang JJ, Zhu JN Histamine H1 Receptor Contributes to Vestibular Compensation. J Neurosci. 2019 Jan 16;39(3):420-433. PMID: 30413645
• Publications:

  Localization of histamine (H1, H2, H3 and H4) receptors in mouse inner ear. Acta Otolaryngol. 136, 537-44 (2016). 26854127

Target Info

• Gene Symbol: Hrh1|HRH1
• Gene Full Name: histamine receptor H 1|histamine receptor H1
• Alias Symbols: Bordetella pertussis induced histamine sensitization;Bp;Bphs;H;H1R;H1-R;HH1R;Hir;hisH1;Hisr;histamine H1 receptor;histamine receptor, subclass H1.
• NCBI Gene Id: 15465|24448|3269
• Protein Name: Histamine H1 receptor
• Uniprot ID: P35367
• Protein Accession #: Variants (NP_000852.1; NP_001091681.1; NP_001091682.1; NP_001091683.1) encode the same protein.
• Molecular Weight: 55.8