HRG ELISA Kit (Human) (OKBB01195)

Catalog No: OKBB01195

Size:96 wells

HRG ELISA Kit (Human) (OKBB01195) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human HRG ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HRG has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: V19-K525） and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HRG is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human HRG amount of sample captured in plate.
• ELISA Kit Range: 156 pg/ml - 10,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	244	1957	4517	246	1967	4596
  Standard deviation	10.49	95.89	243.9	16.48	135.7	344.7
  CV(%)	4.3	4.9	5.4	6.7	6.9	7.5

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Human HRG Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Human HRG Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Human HRG Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	244	264	246	248	250	7.92	3.1%
  Sample . 2	1957	2089	1985	1978	2002	51.13	2.5%%
  Sample 3	4517	4462	4358	4566	4475	77.29	1.7%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	156	313	625	1250	2500	5000	10000
  O.D.	0.131	0.253	0.335	0.536	0.815	1.219	1.671	2.301

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 15 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human HRG
• Immunogen: Expression system for standard: NS0; Immunogen sequence: V19-K525
• Description: For quantitative detection of human HRG in cell culture supernatants, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: HRG
• Gene Full Name: histidine rich glycoprotein
• Alias Symbols: Heme transporter HRG1; Heme-responsive gene 1 protein homolog; HRG-1; hHRG-1; Solute carrier family 48 member 1; SLC48A1; HRG1
• NCBI Gene Id: 3273
• Protein Name: Heme transporter HRG1
• Description of Target: HRG contains two cystatin-like domains and is located in plasma and platelets. The physiological function has not been determined but it is known that the protein binds heme, dyes and divalent metal ions. The encoded protein also has a peptide that displays antimicrobial activity against C. albicans, E. coli, S. aureus, P. aeruginosa, and E. faecalis. It can inhibit rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels.
• Uniprot ID: Q6P1K1
• Protein Accession #: NP_060312.2
• Nucleotide Accession #: NM_017842.2