HPX ELISA Kit (Chicken) (OKCD02592)

Catalog No: OKCD02592

Size:96WELLS

Chicken HPX ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Chicken
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	81-98%	84-97%	95-104%	97-104%
  EDTA Plasma (n=5)	88-95%	95-104%	97-105%	91-99%
  Heparin Plasma (n=5)	80-96%	98-105%	84-92%	87-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hemopexin (HPX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hemopexin (HPX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hemopexin (HPX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hemopexin (HPX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 6.25-400ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	78-103	97
  EDTA Plasma (n=5)	99-105	102
  Heparin Plasma(n=5)	82-97	85

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hemopexin (HPX) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hemopexin (HPX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-HPX Microplate: 96 Wells (12 x 8 Well strips)
  • HPX Lyophilized Standard: 2
  • 100X Biotinylated HPX Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 2.69 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Hemopexin (HPX). No significant cross-reactivity or interference between Hemopexin (HPX) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HPX
• Protein Name: Hemopexin
• Description of Target: Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
• Uniprot ID: P20057
• Protein Size (# AA): 34