Catalog No: OKCD04453
Size:96WELLS
Price: $650.00
SKU
OKCD04453
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Datasheets/ManualsPrintable datasheet for HPT ELISA Kit (Rabbit) (OKCD04453)
Product Info
Predicted Species ReactivityOryctolagus cuniculus|Rabbit
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)84-98%82-92%88-103%83-97%
EDTA Plasma (n=5)88-103%86-101%82-93%89-98%
Heparin Plasma (n=5)81-101%82-99%92-105%84-103%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Haptoglobin (Hpt). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Haptoglobin (Hpt). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Haptoglobin (Hpt), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Haptoglobin (Hpt) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average (%)
Serum (n=5)78-10191
EDTA Plasma (n=5)78-9682
Heparin Plasma (n=5)93-10299
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Haptoglobin (Hpt) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Haptoglobin (Hpt) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Crp Microplate96 Wells (12 x 8 Well strips)
Crp Lyophilized Standard2
100X Crp HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity0.69 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHP
Gene Full Namehaptoglobin
Alias Symbolshaptoglobin;zonulin.
NCBI Gene Id100008816
Protein NameHaptoglobin
Description of TargetAs a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway (By similarity).
Uniprot IDP19007
Protein Accession #NP_001075579.1
Nucleotide Accession #NM_001082110.1
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