HPRT1 ELISA Kit (Pig) (OKEH08155)

Catalog No: OKEH08155

Size:96T

Product Info

• Predicted Species Reactivity: Porcine, Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	107-117% (n = 20)	85-96% (n = 20)	109-119% (n = 20)	110-120% (n = 20)
  EDTA plasma(n=5)	87-97% (n = 20)	106-116% (n = 20)	103-115% (n = 20)	86-97% (n = 20)
  heparin plasma(n=5)	80-89% (n = 20)	103-113% (n = 20)	100-111% (n = 20)	88-101% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology HPRT1 ELISA Kit (Pig) (OKEH08155) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HPRT1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HPRT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HPRT1 captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 91% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.4% (n = 20)
  Mean Inter-assay CV%: <=5.9% (n = 20)
• ELISA Kit Component:
  • HPRT1 Microplate: 96 Wells (12 x 8 Well strips)
  • HPRT1 Lyophilized Standard: 2
  • 100X Biotinylated HPRT1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
  Subcellular Location: Cytoplasm
  Unigene: Ssc.4158
  SMR: Q45FY6
  String: 9823.ENSSSCP00000025410
  KEGG: ssc:397351
  Pfam: PF00156
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Pig Hypoxanthine-guanine phosphoribosyltransferase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HPRT1
• Gene Full Name: hypoxanthine phosphoribosyltransferase 1
• Alias Symbols: HGPRT;HGPRTase;HPRT;hypoxanthine-guanine phosphoribosyltransferase;hypoxanthine-guanine phosphoribosyltransferase 1.
• NCBI Gene Id: 397351
• Protein Name: Q45FY6
• Description of Target: Hypoxanthine-guanine phosphoribosyltransferase
• Uniprot ID: https://www.uniprot.org/uniprot/Q45FY6
• Protein Accession #: NP_001027548.1
• Nucleotide Accession #: NM_001032376.2