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Catalog No: OKEH08152
Size:96T
Price: $800.00
SKU
OKEH08152
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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HPRT1 ELISA Kit (Bovine) (OKEH08152)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology HPRT1 ELISA Kit (Bovine) (OKEH08152) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HPRT1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HPRT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HPRT1 captured in well.
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 112% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=6.4% (n = 20)
Mean Inter-assay CV%: <=8.6% (n = 20)
ELISA Kit Component
ComponentAmount
HPRT1 Microplate96 Wells (12 x 8 Well strips)
HPRT1 Lyophilized Standard2
100X Biotinylated HPRT1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
::Subcellular Location: Cytoplasm
::Unigene: Bt.49238
SMR: Q3SZ18
String: 9913.ENSBTAP00000019547
::KEGG: bta:281229
Pfam: PF00156
Reconstitution and StorageStore as indicated in product manual.
SpecificityNatural and recombinant Bovine Hypoxanthine-guanine phosphoribosyltransferase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHPRT1
Gene Full Namehypoxanthine phosphoribosyltransferase 1
Alias SymbolsHGPRT;HGPRTase;HPRT;hypoxanthine phosphoribosyltransferase 1 (Lesch-Nyhan syndrome);hypoxanthine-guanine phosphoribosyltransferase.
NCBI Gene Id281229
Protein NameQ3SZ18
Description of TargetHypoxanthine-guanine phosphoribosyltransferase
Uniprot IDhttps://www.uniprot.org/uniprot/Q3SZ18
Protein Accession #NP_001029207.1
Nucleotide Accession #NM_001034035.2
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