HPRT1 Antibody - C-terminal region (OAAB03804)

Catalog No: OAAB03804

Size:400ul

Western blot analysis of HPRT1 antibody (C-term) in Hela cell lysates (35ug/lane). HPRT1 (arrow) was detected using the purified Pab.

HPRT1 is strongly supported by BioGPS gene expression data to be expressed in Hela

Western blot analysis of HPRT1 (arrow) using rabbit polyclonal HPRT1 Antibody (C-term) either nontransfected (Lane 1) or lysates transiently transfected transfected (Lane 2) with the HPRT1 gene.

HPRT1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HPRT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

HPRT1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC, FC
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This HPRT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-178 amino acids from the C-terminal region of human HPRT1.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  HPRT1 is strongly supported by BioGPS gene expression data to be expressed in Hela

• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50
• Other Applications Image 1 Data: HPRT1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Publications:

  Göttle, M. et al. Loss of dopamine phenotype among midbrain neurons in Lesch-Nyhan disease. Ann. Neurol. 76, 95-107 (2014). 24891139

Target Info

• Gene Symbol: HPRT1
• Alias Symbols: HPRT, HGPRT
• NCBI Gene Id: 3251
• Protein Name: Hypoxanthine-guanine phosphoribosyltransferase
• Uniprot ID: P00492
• Protein Accession #: NP_000185.1
• Molecular Weight: 24579 Da