HPR Chemi-Luminescent ELISA Kit (Human) (OKCD03563)

Catalog No: OKCD03563

Size:96WELLS

Human HPR ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-97%	83-96%	86-96%	80-90%
  EDTA Plasma (n=5)	97-105%	78-90%	79-95%	85-93%
  Heparin Plasma (n=5)	98-105%	78-99%	81-92%	97-105%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Haptoglobin Related Protein (HPR). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Haptoglobin Related Protein (HPR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Haptoglobin Related Protein (HPR) level in the sample or standard.
• ELISA Kit Range: 68.6-50,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	98-105	101
  EDTA Plasma (n=5)	81-90	84
  Heparin Plasma (n=5)	95-104	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Haptoglobin Related Protein (HPR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Haptoglobin Related Protein (HPR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • HPR Microplate: 96 Wells (12 x 8 Well strips)
  • HPR Lyophilized Standard: 2
  • 100X HPR Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.78 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HPR
• Gene Full Name: haptoglobin-related protein
• Alias Symbols: A-259H10.2;Haptoglobin-related locus;haptoglobin-related protein;HP.
• NCBI Gene Id: 3250
• Protein Name: Haptoglobin-related protein
• Description of Target: Primate-specific plasma protein associated with apolipoprotein L-I (apoL-I)-containing high-density lipoprotein (HDL). This HDL particle, termed trypanosome lytic factor-1 (TLF-1), mediates human innate immune protection against many species of African trypanosomes. Binds hemoglobin with high affinity and may contribute to the clearance of cell-free hemoglobin to allow hepatic recycling of heme iron.
• Uniprot ID: P00739
• Protein Accession #: NP_066275
• Nucleotide Accession #: NM_020995