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Catalog No: OKEH04202
Size:96T
Price: $525.00
SKU
OKEH04202
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Hpgds ELISA Kit (Rat) (OKEH04202) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hpgds has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hpgds is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hpgds captured in the well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit Component
ComponentAmount
Hpgds Microplate96 Wells (12 x 8 Well Strips)
Hpgds Standard2 x 2 ng
Sample Diluent1 x 20 mL
Hpgds Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.092 ng/mL
SpecificityNatural and recombinant Rat Hematopoietic prostaglandin D synthase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHpgds
Gene Full Namehematopoietic prostaglandin D synthase
Alias Symbolsglutathione S-transferase;glutathione-dependent PGD synthase;glutathione-dependent PGD synthetase;glutathione-requiring prostaglandin D synthase;GST class-sigma;hematopoietic prostaglandin D synthase;H-PGDS;prostaglandin D2 synthase 2, hematopoietic;prostaglandin-H2 D-isomerase;Ptgds2.
NCBI Gene Id58962
Protein NameO35543
Description of TargetHematopoietic prostaglandin D synthase
Uniprot IDhttps://www.uniprot.org/uniprot/O35543
Protein Accession #NP_113832.1
Nucleotide Accession #NM_031644.2
Protein Size (# AA)199
Molecular Weight23 kDa
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