Catalog No: OCOA14263
Size:20UG
Price: $179.00
SKU
OCOA14263
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HPD Protein Lysate (OCOA14263)

Datasheets/ManualsPrintable datasheet for HPD Protein Lysate (OCOA14263)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionHPD HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolHPD
Gene Full Name4-hydroxyphenylpyruvate dioxygenase
Alias Symbols4HPPD;4-HPPD;4-hydroxyphenylpyruvate dioxygenase;4-hydroxyphenylpyruvic acid oxidase;GLOD3;glyoxalase domain containing 3;HPPDASE;PPD.
NCBI Gene Id3242
Protein NameNM_002150, NP_002141, P32754
Description of Target4-hydroxyphenylpyruvate dioxygenase, 4-hydroxyphenylpyruvate dioxygenase isoform 1 [Homo sapiens], Homo sapiens 4-hydroxyphenylpyruvate dioxygenase (HPD), transcript variant 1, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/P32754
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_002141
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_002150
Molecular Weight44.9 kDa
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