Catalog No: OKCD01234 (Formerly GWB-KBBLN8)
Size:96WELLS
Price: $675.00
SKU
OKCD01234
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Datasheets/ManualsPrintable datasheet for HPD ELISA Kit (Human) (OKCD01234)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)95-104%84-95%78-99%88-105%
EDTA plasma(n=5)78-90%90-97%95-105%83-97%
heparin plasma(n=5)78-104%93-105%80-92%85-99%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 4-Hydroxyphenylpyruvate Dioxygenase (HPD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 4-Hydroxyphenylpyruvate Dioxygenase (HPD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 4-Hydroxyphenylpyruvate Dioxygenase (HPD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of 4-Hydroxyphenylpyruvate Dioxygenase (HPD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range3.12-200ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)91-9995
EDTA plasma(n=5)90-9995
heparin plasma(n=5)88-9592
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 4-Hydroxyphenylpyruvate Dioxygenase (HPD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 4-Hydroxyphenylpyruvate Dioxygenase (HPD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-HPD Microplate96 Wells (12 x 8 Well Strips)
HPD Lyophilized Standard2 x 50 ng
100X Biotinylated HPD Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.32 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of 4-Hydroxyphenylpyruvate Dioxygenase (HPD). No significant cross-reactivity or interference between 4-Hydroxyphenylpyruvate Dioxygenase (HPD) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolHPD
Gene Full Name4-hydroxyphenylpyruvate dioxygenase
Alias Symbols4HPPD;4-HPPD;4-hydroxyphenylpyruvate dioxygenase;4-hydroxyphenylpyruvic acid oxidase;GLOD3;glyoxalase domain containing 3;HPPDASE;PPD.
NCBI Gene Id3242
Protein Name4-hydroxyphenylpyruvate dioxygenase
Description of TargetKey enzyme in the degradation of tyrosine.
Uniprot IDP32754
Protein Accession #NP_001165464
Nucleotide Accession #NM_001171993.1
Protein Size (# AA)393
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