HP ELISA Kit (Human) (OKGD00002)

Catalog No: OKGD00002

Size:96 Wells

HP ELISA Kit (Human) (OKGD00002) standard curve

Product Info

• Predicted Species Reactivity: Rat, Dog, Pig
• Product Format: Liquid
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~2 hours
• ELISA Kit Principle: Aviva Systems Biology HP ELISA Kit (human) (OKGD00002) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HP has been pre-coated onto a 96-well plate. Standards or test samples are added to the wells, incubated and washed. A horseradish peroxidase (HRP) conjugated detector antibody specific for HP is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HP captured in well.
• ELISA Kit Range: 1.4 - 1000 ng/ml
• ELISA Kit Component:
  • Anti-HP Microplate: 96 Wells (12 x 8 Well strips)
  • HP Lyophilized Standard: 2 vials
  • 100X HP HRP-Detection Anitbody: 1 x 125 uL
  • Assay Diluent: 1 x 30 mL
  • 20X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 12 mL
  • Stop Solution: 1 x 12 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Other Biological Fluids

Target Info

• Gene Symbol: HP
• Gene Full Name: Haptoglobin
• Alias Symbols: BP, HPA1S, HP2ALPHA2
• NCBI Gene Id: 3240
• Protein Name: Haptoglobin
• Description of Target: Haptoglobin is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. The protein encoded also exhibits antimicrobial activity against bacteria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P00738
• Protein Accession #: NP_001119574.1
• Nucleotide Accession #: NM_001126102.2