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Catalog No: OKEH04608
Size:96T
Price: $625.00
SKU
OKEH04608
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH04608
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology HP ELISA Kit (Bovine) (OKEH04608) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for HP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HP captured in well.
ELISA Kit Range7.8-500ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-HP Microplate96 Wells (12 x 8 Well Strips)
HP Lyophilized Standard2
Sample Diluent1 x 20 mL
HP Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity3.4 ng/mL
SpecificityNatural and recombinant Bovine Haptoglobin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHP
Gene Full Namehaptoglobin
Alias Symbolshaptoglobin;zonulin.
NCBI Gene Id280692
Protein NameQ2TBU0
Description of TargetHaptoglobin
Uniprot IDhttps://www.uniprot.org/uniprot/Q2TBU0
Protein Accession #NP_001035560.1
Nucleotide Accession #NM_001040470.2
Protein Size (# AA)401
Molecular Weight45 KDa
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