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Catalog No: OCOA11243
Size:20UG
Price: $179.00
SKU
OCOA11243
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HOXD9 Protein Lysate (OCOA11243)

Datasheets/ManualsPrintable datasheet for HOXD9 Protein Lysate (OCOA11243)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionHOXD9 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolHOXD9
Gene Full Namehomeobox D9
Alias Symbolshomeo box D9;homeobox protein Hox-4C;homeobox protein Hox-5.2;homeobox protein Hox-D9;HOX4;Hox-4.3;Hox-4.3, mouse, homolog of;HOX4C;Hox-5.2.
NCBI Gene Id3235
Protein NameNM_014213, NP_055028, P28356
Description of TargetHomeobox protein Hox-D9, homeobox protein Hox-D9 [Homo sapiens], Homo sapiens homeobox D9 (HOXD9), mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/P28356
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_055028
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_014213
Molecular Weight35.6 kDa
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