Rabbit Anti-Horse IgG (H&L) Polyclonal Antibody - Biotin Conjugated (OARA05323)

Catalog No: OARA05323

Size:2MG

Product Info

• Predicted Species Reactivity: Horse
• Product Format: Lyophilized
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Biotin
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
• Reconstitution and Storage: 2°C to 8°C|-20°C or -80°C
• Immunogen: Horse IgG whole molecule
• Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose.
• Concentration: 2 mg/ml
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Horse IgG and Horse Serum.
• Application Info: ELISA: 1:20,000 - 1:100,000
  WB: 1:2,000 - 1:10,000
  IHC: 1:1,000 - 1:5,000
  This product has been assayed against 1.0 ug of Horse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin OORA00141. A working dilution of 1:14,000 to 1:50,000 of the reconstitution concentration is suggested for this product.
  Optimal dilutions should be determined by the end user.

Target Info

• Alias Symbols: Rabbit Anti-Horse IgG Biotin Conjugated Antibody, Rabbit Anti-Horse IgG Biotin Conjugated Antibody
• Description of Target: Anti-Horse IgG Biotin Antibody generated in rabbit detects horse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
• Molecular Weight: 455