HO-1 Antibody : Biotin (OASE00043-Biotin)

Catalog No: OASE00043-Biotin

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Bovine, Canine, Guinea pig, Hamster, Monkey, Pig, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: 1F12-A6
• Isotype: IgG1 Kappa
• Host: Mouse
• Conjugation: Biotin
• Application: WB, IHC, IP, ICC, ELISA
• Additional Information: Background Info: Detects a 32kDa protein, corresponding to the molecular mass of HO-1 on SDS Page immunoblots. Does not cross-react with HO-2
  Scientific Background: Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2).
  There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).
  
  Certificate of Analysis: 1ug/ml was sufficient for detection of HO-1 in 10ug of mixed human cell line lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Human HO-1 synthetic peptide, amino acids 1-30
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1:500-1000 (WB)
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86.
  2. Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47.
  3. Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411–419.
  4. Brydun A., et al. (2007) Hypertens Res 30(4): 341-8.
  5. Poss K.D. and Tonegawa S. (1997). Proc Natl Acad Sci U S A. 94: 10925–10930.
  6. Yet S.F., et al. (2003) FASEB J. 17: 1759–1761.
  7. Yachie A., et al. (1999) J Clin Invest. 103: 129–135.

Target Info

• Gene Symbol: HMOX1
• Gene Full Name: heme oxygenase (decycling) 1
• Alias Symbols: HO-1, HSP32, HMOX1D, bK286B10
• NCBI Gene Id: 3162
• Uniprot ID: P09601
• Protein Accession #: NP_002124.1