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HNRPF Antibody - C-terminal region (ARP40565_T100)

100 ul
$249.00
In Stock
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Conjugation Options

ARP40565_T100-FITC Conjugated

ARP40565_T100-HRP Conjugated

ARP40565_T100-Biotin Conjugated

Gene Symbol:
HNRNPF
Official Gene Full Name:
Heterogeneous nuclear ribonucleoprotein F
NCBI Gene Id:
3185
Protein Name:
Heterogeneous nuclear ribonucleoprotein F
Swissprot Id:
P52597
Protein Accession #:
NP_004957
Nucleotide Accession #:
NM_004966
Alias Symbols:
HNRPF, mcs94-1, OK/SW-cl.23
Replacement Item:
This antibody may replace item sc-10045 from Santa Cruz Biotechnology.
Description of Target:
HNRPF belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH.This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH.
Protein Size (# AA):
415
Molecular Weight:
46kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Protein A purified
Application:
IHC, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express HNRPF.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express HNRPF.
Immunogen:
The immunogen is a synthetic peptide directed towards the C terminal region of human HNRPF
Predicted Species Reactivity:
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Tested Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Cow: 91%; Dog: 92%; Guinea Pig: 100%; Horse: 92%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
Complete computational species homology data:
Anti-HNRPF (ARP40565_T100)
Peptide Sequence:
Synthetic peptide located within the following region: LNSTTGASNGAYSSQVMQGMGVSAAQATYSGLESQSVSGCYGAGYSGQNS
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
HSFY1; C1orf94; CCDC33; EIF4ENIF1; RBFOX2; IKZF3; HNRNPUL1; HNRNPM; FUS; TFG; DZIP3; UBC; AES; NEDD1; CEP250; SUMO2; SUMO3; TP53; CDC20; STAU1; IVNS1ABP; SUMO1; NEDD8; MDM2; WWOX; RPA3; RPA2; RPA1; ERG; EED; rev; PRPF40A; OSGEP; ABCF3; DNAAF5; PPME1; PPP1
Blocking Peptide:
For anti-HNRNPF (ARP40565_T100) antibody is Catalog # AAP40565 (Previous Catalog # AAPP22743)
Datasheets/Manuals:
Printable datasheet for anti-HNRNPF (ARP40565_T100) antibody
Sample Type Confirmation:

HNRNPF is supported by BioGPS gene expression data to be expressed in HepG2

Target Reference:
Balasubramani,M., (2006) Cancer Res. 66 (2), 763-769
Publications:

White, R. et al. Heterogeneous nuclear ribonucleoprotein (hnRNP) F is a novel component of oligodendroglial RNA transport granules contributing to regulation of myelin basic protein (MBP) synthesis. J. Biol. Chem. 287, 1742-54 (2012). IHC, WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat 22128153

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1 Item(s)

275/10/2019 21:40
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HeLa nuclear extract, pull-down reaction in WB

Submitted by:
Maja Dembic
Syddansk Universitet

HNRPF
Sample type/lane description: HeLa nuclear extract, RNA pull-down reactions done with RNA oligonucleotides and with HeLa nuclear extract.
Lane 1: 10ug HeLa nuclear extract
Lane 2: 10ug protein; RNA pull-down reaction with sequence harboring GGG triplets known to bind hnRNPF/H.
Lane 3: 10ug protein; RNA pull-down reaction with mutated RNA sequence to reduce binding of hnRNPF/H.

Primary antibody dilution: 1:1000 O.N. at 4°C

Secondary antibody dilution: Anti-rabbit 1:10000.

Protocol: Various samples were run and separated on a Bis-Tris 4-12% gradient gel in MOPS 1x
buffer, under denaturing conditions. The samples were transferred on PVDF membrane
(30V constant; 1h, 30 min) and blotted with the antibodies. After blocking for aspecific binding all primary antibodies were incubated O.N., at 4 degrees. Washing and secondary antibody staining was performed on SNAP i.d. 2.0 Protein Detection System, according to instructions. After incubation with ECL, the membranes were exposed for 5 seconds.

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