HNRNPM Antibody - N-terminal region (OAGA04879)

Catalog No: OAGA04879

Size:100 ul

Sample (30 ug of whole cell lysate)
A: 293T
B: HeLa
7.5% SDS PAGE
diluted at 1:10000

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HNRNPM antibody at 1:500 dilution.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using HNRNPM antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled (red) at 1:2000.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human HNRNPM. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:5000-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: HNRNPM
• Gene Full Name: heterogeneous nuclear ribonucleoprotein M
• Alias Symbols: CEAR, HNRPM, HTGR1, NAGR1, HNRPM4, HNRNPM4, hnRNP M
• NCBI Gene Id: 4670
• Protein Name: heterogeneous nuclear ribonucleoprotein M
• Description of Target: This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs. This protein also constitutes a monomer of the N-acetylglucosamine-specific receptor which is postulated to trigger selective recycling of immature GlcNAc-bearing thyroglobulin molecules. Alternative splicing results in multiple transcript variants.
• Uniprot ID: P52272
• Protein Accession #: NP_005959.2
• Nucleotide Accession #: NM_005968.4