Catalog No: OKEH03096
Size:96T
Price: $525.00
SKU
OKEH03096
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology HNRNPK ELISA Kit (Mouse) (OKEH03096) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hnrnpk has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Hnrnpk is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hnrnpk captured in well.
ELISA Kit Range78-5000pg/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 8.4% (n = 20)
Mean Inter-assay CV%: <= 10.5% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Hnrnpk Microplate96 Wells (12 x 8 Well strips)
Hnrnpk Lyophilized Standard2
100X Biotinylated Hnrnpk Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity40 pg/mL
SpecificityNatural and recombinant Mouse Heterogeneous nuclear ribonucleoprotein K
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHnrnpk
Gene Full Nameheterogeneous nuclear ribonucleoprotein K
Alias Symbolsheterogeneous nuclear ribonucleoprotein K;hn;Hnrpk;KBB;KBBP;NOVA.
NCBI Gene Id15387
Protein NameP61979
Description of TargetHeterogeneous nuclear ribonucleoprotein K
Uniprot IDhttps://www.uniprot.org/uniprot/P61979
Protein Accession #NP_001288269.1
Nucleotide Accession #NM_001301340.1
Protein Size (# AA)463
Molecular Weight51 kDa
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