HNRNPF ELISA Kit (Bovine) (OKEH07745)

Catalog No: OKEH07745

Size:96T

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology HNRNPF ELISA Kit (Bovine) (OKEH07745) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HNRNPF has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HNRNPF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HNRNPF captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.1% (n = 20)
  Mean Inter-assay CV%: <=7.1% (n = 20)
• ELISA Kit Component:
  • HNRNPF Microplate: 96 Wells (12 x 8 Well strips)
  • HNRNPF Lyophilized Standard: 2
  • 100X Biotinylated HNRNPF Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.
  Subcellular Location: Nucleus Nucleoplasm
  Unigene: Bt.23170
  SMR: Q5E9J1
  String: 9913.ENSBTAP00000011658
  KEGG: bta:506917
  Pfam: PF00076
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Bovine Heterogeneous nuclear ribonucleoprotein F
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HNRNPF
• Gene Full Name: heterogeneous nuclear ribonucleoprotein F
• Alias Symbols: heterogeneous nuclear ribonucleoprotein F;hnRNP F;HNRPF.
• NCBI Gene Id: 506917
• Protein Name: Q5E9J1
• Description of Target: Heterogeneous nuclear ribonucleoprotein F
• Uniprot ID: https://www.uniprot.org/uniprot/Q5E9J1
• Protein Accession #: NP_001014860.1
• Nucleotide Accession #: NM_001014860.3