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Datasheets/Manuals | Printable datasheet for HNRNPD Antibody (Phospho-Ser83) (OAAF00224) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human hnRPD around the phosphorylation site of Ser83. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | hnRPD (Phospho-Ser83) antibody detects endogenous levels of hnRPD only when phosphorylated at serine 83. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1:500~1:3000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:20000 |
Reference | Kajita Y., J. Biol. Chem. 270:22167-22175(1995). Dempsey L.A., Genomics 49:378-384(1998). Tolnay M., Biochem. J. 338:417-425(1999). Grosset C., Cell 103:29-40(2000) |
Gene Symbol | HNRNPD |
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Gene Full Name | heterogeneous nuclear ribonucleoprotein D |
Alias Symbols | ARE-binding protein AUFI, type A;AUF1;AUF1A;AU-rich element RNA binding protein 1, 37kDa;AU-rich element RNA-binding protein 1;heterogeneous nuclear ribonucleoprotein D0;hnRNP D0;hnRNPD0;HNRPD;P37. |
NCBI Gene Id | 3184 |
Protein Name | Heterogeneous nuclear ribonucleoprotein D0 |
Description of Target | Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Binding of RRM1 to DNA inhibits the formation of DNA quadruplex structure which may play a role in telomere elongation. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. May play a role in the regulation of the rhythmic expression of circadian clock core genes. Directly binds to the 3'UTR of CRY1 mRNA and induces CRY1 rhythmic translation. May also be involved in the regulation of PER2 translation. |
Uniprot ID | Q14103 |
Protein Accession # | NP_001003810.1 |
Molecular Weight | 38 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "HNRNPD Antibody (Phospho-Ser83) (OAAF00224)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "HNRNPD Antibody (Phospho-Ser83) (OAAF00224)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "HNRNPD Antibody (Phospho-Ser83) (OAAF00224)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "HNRNPD Antibody (Phospho-Ser83) (OAAF00224)"?
This target may also be called "ARE-binding protein AUFI, type A;AUF1;AUF1A;AU-rich element RNA binding protein 1, 37kDa;AU-rich element RNA-binding protein 1;heterogeneous nuclear ribonucleoprotein D0;hnRNP D0;hnRNPD0;HNRPD;P37." in publications.
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What is the shipping cost for "HNRNPD Antibody (Phospho-Ser83) (OAAF00224)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "HNRNPD Antibody (Phospho-Ser83) (OAAF00224)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "HNRNPD Antibody (Phospho-Ser83) (OAAF00224)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "38 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "HNRNPD Antibody (Phospho-Ser83) (OAAF00224)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "HNRNPD"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "HNRNPD"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "HNRNPD"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "HNRNPD"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "HNRNPD"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "HNRNPD"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.