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Catalog No: OCOA04637
Size:20UG
Price: $275.00
SKU
OCOA04637
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HNF4A Protein Lysate (OCOA04637)

Datasheets/ManualsPrintable datasheet for HNF4A Protein Lysate (OCOA04637)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionHNF4A HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolHNF4A
Gene Full Namehepatocyte nuclear factor 4 alpha
Alias SymbolsFRTS4;hepatic nuclear factor 4 alpha;hepatocyte nuclear factor 4-alpha;HNF4;HNF4a7;HNF4a8;HNF4a9;HNF4alpha;HNF4alpha10/11/12;MODY;MODY1;NR2A1;NR2A21;nuclear receptor subfamily 2 group A member 1;TCF;TCF14;TCF-14;transcription factor 14;transcription factor HNF-4.
NCBI Gene Id3172
Protein NameNM_178850, NP_849181, P41235
Description of TargetHepatocyte nuclear factor 4-alpha, hepatocyte nuclear factor 4-alpha isoform 3 [Homo sapiens], Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant 3, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/P41235
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_849181
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_178850
Molecular Weight46.4 kDa
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