- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for HNF4A Antibody (OAEB01754) |
|---|
| Predicted Species Reactivity | Dog|Human|Mouse|Porcine |
|---|---|
| Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Clonality | Polyclonal |
| Host | Goat |
| Application | WB, IHC |
| Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
| Immunogen | HNF4A |
| Purification | Ammonium Sulfate Precipitated; Affinity Purified |
| Predicted Homology Based on Immunogen Sequence | Human, Dog, Pig |
| Peptide Sequence | RLSKTLVDMDMADY-C |
| Concentration | 0.5 mg/ml |
| Specificity | This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1; NP_000448.3; NP_849181.1 resp.). |
| Application Info | P-ELISA: Antibody detection limit dilution 1:32000 WB: Approx 55 kDa and 48 kDa bands observed in Mouse Small Intestine and in lysates of cell line HepG2 (calculated MW of 52.8 kDa according to Human NP_000448.3 , 46.6 kDa according to Human NP_849181.1, 52.7 kDa according to Mouse NP_032287.2 and 49.9 kDa according to Mouse NP_001299835.1). Recommended concentration: 0.1-0.5 ug/ml. Primary incubation was 1 hour. IHC: Paraffin embedded Human Small Intestine. Recommended concentration: 2 ug/ml. |
| Reference | * Damcott CM, Hoppman N, Ott SH, Reinhart LJ, Wang J, Pollin TI, O'Connell JR, Mitchell BD, Shuldiner AR.; Polymorphisms in both promoters of hepatocyte nuclear factor 4-alpha are associated with type 2 diabetes in the Amish.; Diabetes. 2004 Dec;53(12):3337-41.; Pubmed 15561969 |
| Gene Symbol | HNF4A |
|---|---|
| Gene Full Name | hepatocyte nuclear factor 4 alpha |
| Alias Symbols | FRTS4;hepatic nuclear factor 4 alpha;hepatocyte nuclear factor 4-alpha;HNF4;HNF4a7;HNF4a8;HNF4a9;HNF4alpha;HNF4alpha10/11/12;MODY;MODY1;NR2A1;NR2A21;nuclear receptor subfamily 2 group A member 1;TCF;TCF14;TCF-14;transcription factor 14;transcription factor HNF-4. |
| NCBI Gene Id | 3172 |
| Protein Name | Hepatocyte nuclear factor 4-alpha |
| Uniprot ID | P41235 |
| Protein Accession # | NP_849180.1 |
| Molecular Weight | 51.6; 52.8; 46.6 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "HNF4A Antibody (OAEB01754)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Dog|Human|Mouse|Porcine".
-
How long will it take to receive "HNF4A Antibody (OAEB01754)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
-
What buffer format is "HNF4A Antibody (OAEB01754)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "HNF4A Antibody (OAEB01754)"?
This target may also be called "FRTS4;hepatic nuclear factor 4 alpha;hepatocyte nuclear factor 4-alpha;HNF4;HNF4a7;HNF4a8;HNF4a9;HNF4alpha;HNF4alpha10/11/12;MODY;MODY1;NR2A1;NR2A21;nuclear receptor subfamily 2 group A member 1;TCF;TCF14;TCF-14;transcription factor 14;transcription factor HNF-4." in publications.
-
What is the shipping cost for "HNF4A Antibody (OAEB01754)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "HNF4A Antibody (OAEB01754)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "HNF4A Antibody (OAEB01754)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "51.6; 52.8; 46.6".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "HNF4A Antibody (OAEB01754)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "HNF4A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "HNF4A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "HNF4A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "HNF4A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "HNF4A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "HNF4A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
