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Datasheets/Manuals | Printable datasheet for HNF4 alpha Antibody (Phospho-Ser313) (OAAF07723) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Additional Information | Modification Sites: Human:S313 Mouse:S313 Rat:S313 |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human HNF4 alpha around the phosphorylation site of Ser313. |
Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
Peptide Sequence | Synthetic peptide located within the following region: QIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYD |
Concentration | 1mg/ml |
Specificity | HNF4 alpha (Phospho-Ser313) Antibody detects endogenous levels of HNF4 alpha only when phosphorylated at Ser313. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Application Info | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 |
Gene Symbol | HNF4A |
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Gene Full Name | hepatocyte nuclear factor 4 alpha |
Alias Symbols | FRTS4;hepatic nuclear factor 4 alpha;hepatocyte nuclear factor 4-alpha;HNF4;HNF4a7;HNF4a8;HNF4a9;HNF4alpha;HNF4alpha10/11/12;MODY;MODY1;NR2A1;NR2A21;nuclear receptor subfamily 2 group A member 1;TCF;TCF14;TCF-14;transcription factor 14;transcription factor HNF-4. |
NCBI Gene Id | 3172 |
Protein Name | Hepatocyte nuclear factor 4-alpha |
Description of Target | Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine. |
Uniprot ID | P41235 |
Molecular Weight | 52 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "HNF4 alpha Antibody (Phospho-Ser313) (OAAF07723)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "HNF4 alpha Antibody (Phospho-Ser313) (OAAF07723)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "HNF4 alpha Antibody (Phospho-Ser313) (OAAF07723)" provided in?
This item is provided in "".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "HNF4 alpha Antibody (Phospho-Ser313) (OAAF07723)"?
This target may also be called "FRTS4;hepatic nuclear factor 4 alpha;hepatocyte nuclear factor 4-alpha;HNF4;HNF4a7;HNF4a8;HNF4a9;HNF4alpha;HNF4alpha10/11/12;MODY;MODY1;NR2A1;NR2A21;nuclear receptor subfamily 2 group A member 1;TCF;TCF14;TCF-14;transcription factor 14;transcription factor HNF-4." in publications.
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What is the shipping cost for "HNF4 alpha Antibody (Phospho-Ser313) (OAAF07723)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "HNF4 alpha Antibody (Phospho-Ser313) (OAAF07723)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "HNF4 alpha Antibody (Phospho-Ser313) (OAAF07723)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "52 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "HNF4 alpha Antibody (Phospho-Ser313) (OAAF07723)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "HNF4A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "HNF4A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "HNF4A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "HNF4A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "HNF4A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "HNF4A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.