HNF1B ELISA Kit (Human) (OKCD08978)

Catalog No: OKCD08978

Size:96WELLS

HNF1B ELISA Kit (Human) (OKCD08978) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Nuclear Factor 1 Beta (HNF1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepatocyte Nuclear Factor 1 Beta (HNF1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatocyte Nuclear Factor 1 Beta (HNF1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hepatocyte Nuclear Factor 1 Beta (HNF1b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Beta (HNF1b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Beta (HNF1b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • HNF1B Microplate: 96 Wells (12 x 8 Well strips)
  • HNF1B Lyophilized Standard: 2
  • 100X HNF1B HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.047ng/mL

Target Info

• Gene Symbol: HNF1B
• Gene Full Name: HNF1 homeobox B
• Alias Symbols: ADTKD3;FJHN;hepatocyte nuclear factor 1-beta;HNF1 beta A;HNF-1B;HNF1beta;HNF-1-beta;HNF2;homeoprotein LFB3;HPC11;LFB3;LF-B3;MODY5;RCAD;T2D;TCF2;TCF-2;Transcription factor 2;transcription factor 2, hepatic;Variant hepatic nuclear factor 1;VHNF1.
• NCBI Gene Id: 6928
• Protein Name: Hepatocyte nuclear factor 1-beta
• Description of Target: Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
• Uniprot ID: P35680
• Protein Accession #: NP_000449.1
• Nucleotide Accession #: NM_000458.3